Chris Pratt Mario (voice) Anya Taylor-Joy Princess Peach (voice) Charlie Day Luigi (voice) Jack Black Bowser (voice) Keegan-Michael Key Toad (voice) Seth Rogen Donkey Kong (voice) Fred Armisen Cranky Kong (voice) Sebastian Maniscalco Spike (voice) Charles Martinet Mario's Dad / Giuseppe (voice) Kevin Michael Richardson Kamek (voice) Khary Payton Penguin King (voice) Rino Romano Uncle Tony (voice) John DiMaggio Uncle Arthur (voice) Jessica DiCicco Mario's Mom (voice) Eric Bauza Toad General (voice) Scott Menville Koopa General (voice) Carlos Alazraqui Additional Voices (voice) Ashly Burch Additional Voices (voice) Rachel Butera Additional Voices (voice) Cathy Cavadini Additional Voices (voice) Will Collyer Additional Voices (voice) Phil LaMarr Additional Voices (voice) Cree Summer Additional Voices (voice)